Thursday, February 18, 2010
Top picture shows a gel electrophoresis chamber that came with the Discovery DNA Kit. The CSI DNA kit has one as well. The chamber uses 3 - 9 volt batteries. The gel is made using 9 ml of agar or agarose. It makes 3 wells for loading and runs from negative to positive. It is a nice little chamber and works well.
Here in the lower picture I bring together all the items I will need to put together a gel electrophoresis chamber of my own. This will allow me to add more wells and to place the wells in different spots on the gel.
I have a plastic bin for the chamber(this holds everything in place, the gel, sample, buffer and electrodes). I will use a plastic lozenges box for the gel chamber (you can use a soap dish or any other dish that will fit into the main chamber or box). You cut the ends off the lozenges box and use either masking tape or I will try and use Glad press and seal on the ends to form the gel. The styrofoam will be used to make a comb to form the wells in the gel. I will cut out a few different ones. The aluminum will be placed on the inside sides of the large box on opposite ends and will be used as electrodes. I will look for some stainless steal nails or screws to replace the aluminum.
To power the electrophoresis I will be using a old model train transformer that I found and repaired. It goes from 0 - 21 volts, not nearly enough voltage but it will do until I can come up with something better. Or I could use 3-5 9 volt batteries for the power supply.
You can see a couple of packages of agar-agar that I found at an Asian store. These will be used for the gel. I do have some powdered agarose (but not much about 1.5 gm) that I will compare with the agar-agar in my tests.
Here are some web sites that may help in constructing the gel electrophoresis: