3D Printing

Electrophoresis Chamber

Dremelfuge

Vessel Holders

3D Printing

3D printing is becoming more accessible to the average person. Maker spaces usually have one or two that would allow you to use. 3D printing is another good way to make lab equipment, here are a few items I have printed:

                   

In the top picture you will see a electrophoresis chamber made from a soap dish and 3D printed gel chamber and comb. I could have printed the whole chamber but I used a soap dish that I already had to save on plastic.

In the next picture I printed the dremelfuge which I have talked about in an earlier post. I printed both with chuck and with-out. So far I only used it with a drill. I still do not feel safe using it with a Dremel without enclosing it in some sort of housing to protect myself.

The last picture shows vessel holders for beakers, flasks and jars that I will attach to a shaker machine. I will talk about this in a later post.

You can find these items at :

http://www.thingiverse.com/

There are many other sites you can get 3D objects from.

New Centrifuge From the Old

New Incubator

I found this inexpensive centrifuge and brought it home to find it not working. I took it apart and cleaned it up. It only needed rewiring to get it up and running. Now with this I am able to retire my blenderfuge. In the blenderfuge the center piece kept warping and had to be replaced. I believe this was caused by the very high speeds of the blender. The blenderfuge was much faster than this centrifuge but this one will work for my needs.

DremelFuge - centrifuge

Warning - be careful when using or building a centrifuge, they are very dangerous. If at all possible try and buy a professional one.

Here is a centrifuge created by Cathal Garvey called DremelFuge. It can be found here:

http://www.thingiverse.com/thing:1483

It uses standard 1.5ml/2ml Eppendorf/Microcentrifuge tubes. Works with both a drill or Dremel.

DremelFuge

I was trying to get it printed out but I was having trouble with it. So I decided I would try to make my own from parts laying around. It consists of a 3.5 inch PVC plumbing cap and a bolt, nut and washer. As seen below I have it running on a drill press. I did try it on a dremel, it worked, but I would not use it that way unless a closed off compartent is built for it (later project).

Homemade dremelfuge

Working in drill press

Centrifuge Revisited

I have been asked to talk more about how I put together my centrifuge.

In the first picture the centrifuge is partly disassembled. The blender, 2 large salad bowls, small metal mixing bowl, 2 toothbrush travel holders(tops), 2 small angle bars with 1 hole at each end, 4 screws, nuts and lock washers and 2 plastic adjustable ties, 4 clips(to hold salad bowls together when spinning), fishing weights and foam(for inside bottom of tube holders not seen) and duct tape.

The second shows where I cut the upper wall of the blender to flatten and level out the top so the metal dish can spin without touching anything.

In the third picture I put the tube holder put together. I measured the height of the side hole(this is important must be the same for both holders) and drilled this hole slightly larger then the screw. I screwed the angle iron on the tube holder with the screw head on the inside so that it is flat on the inside of the holder. I used a lock washer to tighten it properly. Then I used a plastic adjustable tie to support and steady the tube. Then I did the same for the second holder. Using a balance, some foam and a bit of lead(fishing weights) I balanced each one with the other so they offset each other.

In picture 4 I drilled a hole in the center of the metal dish. This must be done carefully because if not exactly centered it will wobble. First time I used a drill and vise to hold the bowl and did not get it right. Second time I used a drill press and a drill press vise to center the bowl and this time it worked. Now place this on the blender and make sure it spins evenly before going on to the next step. Next you have to drill the side holes for the tube holders(again use a drill press and vise if possible to keep everything steady, you don't want the holes to be of different sizes this could add a weight imbalance by taking off too much from 1 side than the other) . Again you must measure correctly to offset each holder on opposite ends. Now place this on the blender and make sure it spins evenly before going on to the next step. Now put the bowl and holders together.

Next I cut out a large hole in the center of one of the salad bowls. This will fit over and sit on the blender. I used duct tape to hold the bowl in place. Now I placed the tube holder and small bowl on the blender and secured it in place with the blender center piece(shown in the third picture). I placed the 2nd salad bowl on top of the 1st salad bowl edge to edge with the bottom of the bowl pointed up. I used the clips to hold it in place.

Then I tried it out. I do not recommend trying this with out the salad bowls in place. If anything goes flying this should hopefully protect you. Remember be careful when building and using a centrifuge they can be very dangerous. So create at your own risk and I am not responsible for any injuries from building or using a homemade centrifuge.