Book: Zero to Genetic Engineering Hero:

Zero to Genetic Engineering Hero: The Beginner's Guide To Programming Bacteria At Home, School and In the Makerspace.

By Justin Pahara and Julie Legault.

This is a newer book. As well as discussing older books I will be looking at newer books to. I just received this book from Amazon. It is a very interesting book. As the title states it takes you from beginning basics to the understanding of bio-engineering. There are at least 9 lab exercises to do. From DNA extraction to manually turning on genes.

It has a section called "Setting up your Genetic Engineering Hero Space". Covers bio-safety and governmental regulations. The book has sections giving you the background information of what is happening and why.

Other than the first exercise, DNA extraction, the other exercises require different kits from the supplier of the book. This can get costly but each kit can be purchased separately, which may help in keeping the costs down. It seems the kits have everything you need to do the experiments easily. The hardware is extra - incubator, water bath, etc.

It also requires a lab hardware kit, called, "The DNA Playground Minilab". It incubates, cools and heat shocks the material. This can be costly. But if you follow this blog most of the hardware needed you can build yourself.  

I will see if I can get some of the lab exercise kits and try them out. I will discuss this in a later post.

Even More Books

Modern Book

Older Books

I have picked up 3 more books for my library. 2 are older and from a better time for citizen science. The other one newer but important for the amateur scientist. (I truly dislike using amateur or citizen scientist. It gives the impression that real science can not be done by them.)

1) Apparatus - 1967 by F. F. Blackwell.

This book shows you how to build various science equipment from household materials. Focusing more on the physical sciences but can be applied to the biological as well. Sections include:

Elementary electricity, Biological apparatus, Light, Machines, Air, Sound and heat, Water, Weather and soil studies.

2) Procedures in Experimental Physics - by John Strong. Originally published in 1938 republished 1986.

Another book dealing with physics. It has great explanations of certain techniques. This book and The Amateur Scientist by C.L. Stong make for great physics experiments of old. Sections include:

Glass blowing, High Vacuum technique, Optical work, Photoelectric cells, Heat and high temperature, Molds and casting, etc.

3) The Laboratory Companion: A Practical Guide to Materials Equipment and Technique - by Gary S. Coyne 2006.

This is a very useful book that discusses the different materials used in labs and how to maintain them. This is a modern book discussing modern equipment.

When I look at some of the older books I pick up I can't believe how many books existed that helped young people build their own labs and equipment. A far cry from what you find today. I am glad that I am still able to find some of them.

More Books

I have picked up a couple of interesting books, a couple of older 1960's books and one a little newer late 1990's:

1) Junior Science Projects - 1967 by the Editors of Science Experimenter:

This book has many experiments you can do and many pieces of equipment that you can build. Such as a milli-microampere detector, ion-exchange fuel cell, diffraction grating-type spectrscope, thermistor thermometer, make your own plastic lab equipment (see picture above) and more. Some of the electronic projects require specific tubes, but I am sure with internet searches you can find other ways to make them. A real interesting read.

2) Exploring Science in your Home Laboratory - 1963 by Richard Harbeck:

This book discusses how to build a home science laboratory for chemistry, biology, physics and geology. It walks you through choosing where to locate the lab, which one you would like to build first. Then helps you set it up, from building a work bench with shelves and then a way to remove the air if you don't have a window in your area. From here it helps you build different lab equipment for your lab, such as a simple electric heater, alcohol lamp, drying and sterilizing oven, test tube racks, stands, beam balance and more. This book must have been a real gem back in it's day.

3) A Low Cost Approach to PCR - 1998 by Eva Harris:

This is a good book for the DIYBIO groups. The low cost approach outlined in this book can be useful for the DIYBIO group. It provides a description of the theoretical basis of the technique and practical details of the method. There is a section on materials and chemicals needed in your lab. A section on building your own equipment ie. electohoresis chamber, micro centrifuge, etc. A section on preparing your reagents. I believe a very useful book for the DIYBIO person.

More Of My Lab

More of My Lab

This picture shows more of my lab. Most of my biological work gets done here.

Over the summer I have picked up many discarded items:

VCR's - they have motors that can move things and timers to set things up.
Blenders, slow cookers (maybe able to use for water baths), electric cup warmers (again use for water baths), aquarium and aquarium heaters.

A great book I found a bit out-dated: Recombinant DNA a Short Course by James Watson, John Tooze and David Kurtz. It is a short course on DNA manipulation, so far a very interesting read. I found this at a second hand book store for $1.00.

Another book I have read over the summer was "A Life Decoded" by Craig Venter. It deals with Venter's time dealing with the human genome group, as well as other parts of his life.

If you want plenty to read and watch, go to www.hhmi.org. There is so much there, so much information. You can even download their journal as PDF files. They have interactive labs, etc.

Books

It has been awhile since I added any information. I have spent some time in building my incubator. Testing it out exactly. I will be placing the fish tank heater into a glass dish of water to heat the incubator. I tested this out and it seems to work quite well. I will add either some copper sulphate or aquarium anti-microbial solution to the water to keep microbial life from growing in the glass dish. I need to come up with some shelves to hold plates, tubes and flasks at different heights in the incubator. I will post a final picture of the incubator when it is finished.

For father's day I received a wonderful book titled: Build-it-Yourself Science Laboratory by Raymond E. Barrett. It was published in 1963. It shows you how to build all kinds of lab equipment, for chemistry, physics, and biology. It even has questions to answer by scientific experimentation after each section. We need a book like this today for building our own labs.

Other books I have picked up for my growing library:

Magic with Chemistry by Edward L. Palder 1964.

150 Science Experiments Step-By-Step by Judith Viorst 1963.

Physics, Fun and Beyond by Eduardo de Campos Valadares 2006.

The Thomas Edison book of Easy and Incredible Experiments by the Thomas Alva Edison Foundation 1988.

Basic Chemistry Experiments: A Golden Hobby Book by Robert Brent 1965 edition.

So now I have many more books to add to my collection and more experiments to try out.